Expression of PCGEM1 and lincRNA-p21 lncRNAs in breast cancer tissue
Abstract
Objectives: Long non-coding RNAs (lncRNAs) play critical roles in tumor development, progression, and prognosis. Prostate cancer gene expression marker 1 (PCGEM1) is an oncogenic lncRNA that inhibits apoptosis, whereas lincRNA-p21 promotes apoptosis. This study aimed to investigate the expression of these lncRNAs in breast tumor tissue and adjacent non-tumor tissue.
Methods: The expression levels of PCGEM1 and lincRNA-p21 were assessed by real-time PCR (RT-PCR) in fifty formalin-fixed, paraffin-embedded invasive ductal carcinoma breast tumor tissues and adjacent non-tumor tissues.
Results: The expression of PCGEM1 was significantly higher in tumor tissues compared to adjacent non-tumor tissues (p=0.01). ROC curve analysis indicated that the sensitivity and specificity of PCGEM1 for discriminating breast cancer were %60.87 and 70.21%, respectively, with an area under the curve (AUC) of 0.63 (p=0.01). No significant correlation was observed between PCGEM1 expression and tumor size, histological grade, or lymph node involvement. The expression of lincRNA-p21 was similar in tumor tissues and adjacent non-tumor tissues.
Conclusions: PCGEM1 may contribute to the pathogenesis of breast cancer and could serve as a diagnostic marker in breast cancer.
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23. Hsieh F-C, Cheng G, Lin J. Evaluation of potential Stat3-regulated genes in human breast cancer. Biochem Biophys Res Commun. 2005;335(2):292-9. https://doi.org/10.1016/j.bbrc.2005.07.075
2. Ehsan N, Zohreh A, Banafsheh S, Hannaneh Z, Yousef M, Marzieh S, et al. The expression of sirtuins in breast cancer and their prognostic and clinicopathological significance: a systematic review. Acta Biochim Iran. 2024;2(2). https://doi.org/10.18502/abi.v2i2.17932.
3. Cerk S, Schwarzenbacher D, Adiprasito JB, Stotz M, Hutterer GC, Gerger A, et al. Current status of long non-coding RNAs in human breast cancer. Int J Mol Sci. 2016;17(9):1485. https://doi.org/10.3390/ijms17091485
4. Zhang Y, Zhang K, Luo Z, Liu L, Wu L, Liu J. Circulating long non‐coding HOX transcript antisense intergenic ribonucleic acid in plasma as a potential biomarker for diagnosis of breast cancer. Thorac cancer. 2016;7(6):627-32. https://doi.org/10.1111/1759-7714.12373
5. Li J, Meng H, Bai Y, Wang K. Regulation of lncRNA and its role in cancer metastasis. Onco Res. 2016;23(5):205. https://doi.org/10.3727/096504016x14549667334007
6. Liu Y, Sharma S, Watabe K. Roles of lncRNA in breast cancer. Front Biosci. 2015;7:94. https://doi.org/10.2741/427
7. Su Y, Gu X, Zheng Q, Zhu L, Lu J, Li L. LncRNA PCGEM1 in human cancers: functions, mechanisms and promising clinical utility. Front Oncol. 2022;12:847745. https://doi.org/10.3389/fonc.2022.847745
8. Ghafouri-Fard S, Khoshbakht T, Hussen BM, Baniahmad A, Taheri M, Mokhtari M. A review on the role of PCGEM1 lncRNA in cancer. Pathol Res Pract. 2022;240:154223. https://doi.org/10.1016/j.prp.2022.154223
9. Qiao F, Wei Z, Jun Y, Zhi H, Zhichao Z, Wei S, et al. Expression of lncRNA PCGEM1 in prostate cancer and its effects on proliferation, apoptosis, invasion, migration and epithelial mesenchymal transition of prostate cancer PC3 cells. J Clin Urol. 2023;38(11):856-61.
10. Chen S, Liang H, Yang H, Zhou K, Xu L, Liu J, et al. LincRNa-p21: function and mechanism in cancer. Med Oncol. 2017;34(5):98. https://doi.org/10.1007/s12032-017-0959-5
11. Huang Y, Yi Q, Feng J, Xie W, Sun W, Sun W. The role of lincRNA-p21 in regulating the biology of cancer cells. Hum Cell. 2022;35(6):1640-9. https://doi.org/10.1007/s13577-022-00768-4
12. Dimitrova N, Zamudio JR, Jong RM, Soukup D, Resnick R, Sarma K, et al. LincRNA-p21 activates p21 in cis to promote Polycomb target gene expression and to enforce the G1/S checkpoint. Mol Cell. 2014;54(5):777-90. https://doi.org/10.1016/j.molcel.2014.04.025
13. Jia M, Jiang L, Wang YD, Huang JZ, Yu M, Xue HZ. lincRNA‐p21 inhibits invasion and metastasis of hepatocellular carcinoma through Notch signaling‐induced epithelial–mesenchymal transition. Hepatol Res. 2016;46(11):1137-44. https://doi.org/10.1111/hepr.12659
14. Jin S, Yang X, Li J, Yang W, Ma H, Zhang Z. p53-targeted lincRNA-p21 acts as a tumor suppressor by inhibiting JAK2/STAT3 signaling pathways in head and neck squamous cell carcinoma. Mol Cancer. 2019;18(1):38. https://doi.org/10.1186/s12943-019-0993-3
15. Afifi AM, Saad AM, Al‐Husseini MJ, Elmehrath AO, Northfelt DW, Sonbol MB. Causes of death after breast cancer diagnosis: A US population‐based analysis. Cancer. 2020;126(7):1559-67. https://doi.org/10.1002/cncr.32648
16. Srikantan V, Zou Z, Petrovics G, Xu L, Augustus M, Davis L, et al. PCGEM1, a prostate-specific gene, is overexpressed in prostate cancer. Proc Natl Acad Sci USA. 2000;97(22):12216-21. https://doi.org/10.1073/pnas.97.22.12216
17. Chen S, Wang L-l, Sun K-x, Liu Y, Guan X, Zong Z-h, Zhao Y. LncRNA PCGEM1 induces ovarian carcinoma tumorigenesis and progression through RhoA pathway. Cell Physiol Biochem. 2018;47(4):1578-88. https://doi.org/10.1159/000490931
18. Guan B, Chen F, Wu Z, Wang C, Yang J. lncRNA PCGEM1 Regulates the Progress of Colorectal Cancer through Targeting miR‐129‐5p/SOX4. Dis Markers. 2022;2022(1):2876170. https://doi.org/10.1155/2022/2876170
19. Li Q, Shen F, Zhao L. The relationship between lncRNA PCGEM1 and STAT3 during the occurrence and development of endometrial carcinoma. Biomed Pharmacother. 2018;107:918-28. https://doi.org/10.1016/j.biopha.2018.08.091
20. Zhang J, Jin H, Wu Y, Zheng Z, Guo S, Wang Y, et al. Hypoxia-induced LncRNA PCGEM1 promotes invasion and metastasis of gastric cancer through regulating SNAI1. Clin Transl Oncol. 2019;21(9):1142-51. https://doi.org/10.1007/s12094-019-02035-9
21. Zhang Q, Zheng J, Liu L. The long noncoding RNA PCGEM1 promotes cell proliferation, migration and invasion via targeting the miR-182/FBXW11 axis in cervical cancer. Cancer Cell Int. 2019;19(1):304. https://doi.org/10.1186/s12935-019-1030-8
22. Fagan‐Solis KD, Schneider SS, Pentecost BT, Bentley BA, Otis CN, Gierthy JF, Arcaro KF. The RhoA pathway mediates MMP‐2 and MMP‐9‐independent invasive behavior in a triple‐negative breast cancer cell line. J Cell Biochem. 2013;114(6):1385-94. https://doi.org/10.1002/jcb.24480
23. Hsieh F-C, Cheng G, Lin J. Evaluation of potential Stat3-regulated genes in human breast cancer. Biochem Biophys Res Commun. 2005;335(2):292-9. https://doi.org/10.1016/j.bbrc.2005.07.075
| Files | ||
| Issue | Vol 3 No 3 (2025) | |
| Section | Original Articles | |
| Keywords | ||
| Long non-coding RNAs (lncRNAs) Prostate cancer gene expression marker 1 (PCGEM1) lincRNA-p21 Breast cancer | ||
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How to Cite
1.
Mohammadhosseini M, Shayanfar N, Sharifi R. Expression of PCGEM1 and lincRNA-p21 lncRNAs in breast cancer tissue. ABI. 2025;3(3):150-154.
