Original Articles

Glutathione Peroxidase 1 Pro198Leu Polymorphism and Susceptibility to Rheumatoid Arthritis: Evidence from an Iranian Case-Control Study

Abstract

Objective: Oxidative stress plays a key role in the pathogenesis of rheumatoid arthritis (RA). Glutathione peroxidase (GPx) is a peroxidase enzyme that defends mammalian cells against oxidative stress.  The Pro198Leu polymorphism of the GPx-1 gene, has been reported to influence its activity. This study aimed to investigate the association of this polymorphism with RA risk in an Iranian population.

Methods: In this case-control study, 45 RA patients and 45 healthy controls were enrolled. Genomic DNA was extracted from blood samples, and genotyping for the Pro198Leu polymorphism was performed using the PCR-RFLP technique. Statistical analysis was conducted using SPSS software, employing chi-square tests and ANOVA.

Results: The mean age of all participants was 55.69 years. Genotype distribution was as follows: CC (50.0%), CT (44.4%), and TT (5.6%). Chi-square analysis revealed no significant difference in genotype frequencies between patients and controls (p = 0.779). Furthermore, no significant association was found between genotypes and age or gender. All groups were in Hardy-Weinberg equilibrium.

Conclusion: The findings indicate that the GPx-1 Pro198Leu polymorphism is not significantly associated with the risk of developing RA in the studied population.

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Keywords
Rheumatoid Arthritis GPx-1 Pro198Leu Single Nucleotide Polymorphism
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How to Cite
1.
Abbaszadeh A, Asgharzadeh MR, Gholinejad Z. Glutathione Peroxidase 1 Pro198Leu Polymorphism and Susceptibility to Rheumatoid Arthritis: Evidence from an Iranian Case-Control Study. ABI. 2025;3(4):215-220.