Tehran University of Medical Sciences Acta Biochimica Iranica 0001-5261 2 1 2024 03 22 31 35 EN Narges torkashvand Tehran university of medical science Haniyeh Kamyab Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Ahmad Reza Shahverdi Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Reza Khoshayand Department of Food and Drug Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Amir Karimi Tarshizi Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran Zargham Sepehrizadeh Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran. 2024 01 30 2024 02 17 Genome analysis of bacteriophages is crucial for their successful application in clinical and biocontrol settings. In this study, we isolated a new lytic phage, vB_SenS_TUMS-E15, from hospital sewage against Salmonella enteritidis and analyzed its genomic features. Complete genome analysis revealed that E15 had circularly permuted double-stranded DNA of 43,048 base pair (bp), with a G+C content of 49.7%. Sixty coding sequences (CDSs) were predicted in the genome, with 44 CDSs encoding known proteins in different modules, including the packaging, structure, replication and metabolism, and lysis modules, and there were no tRNA genes in the genome. Eight transcriptional promoter sequences and 37 rho-independent terminators were detected in the E15 genome. Phylogenetic analysis based on whole-genome sequences suggested that phage E15 should be included as a member of the Jersyvirus genus in the subfamily Guernseyvirinae. Also, no antibiotic-resistance genes, toxins, virulence factors, or lysogen-forming genes were observed in the genome. This indicates that E15 is a lytic phage, making it a promising candidate for clinical and biocontrol purposes. https://abi.tums.ac.ir/index.php/abi/article/view/75 https://abi.tums.ac.ir/index.php/abi/article/download/75/48